ChromoTek RFP-Trap Agarose
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
Specificity
mCherry, mRFP, mPlum
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
218
Cat no : rta
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 5 nM |
| Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
| Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Binding capacity | 10 µL slurry bind ~ 9 µg of recombinant mCherry |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
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| SDS RFP-Trap Agarose (PDF) |
| Protocol |
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| Protocol RFP-Trap Agarose (PDF) |
| RFP-Trap Specificity |
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| Fluorescent protein specificity table (PDF) |
| Brochure |
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| Product brochure (PDF) |
| Application notes |
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| Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
| Split Fluorescent Protein Technology (PDF) |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (IP) (PDF) |
Publications
| Application | Title |
|---|---|
mBio The Unfolded Protein Response Regulates Pathogenic Development of Ustilago maydis by Rok1-Dependent Inhibition of Mating-Type Signaling. | |
Mol Autism Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus. | |
mBio PAS Domain Protein Pas3 Interacts with the Chromatin Modifier Bre1 in Regulating Cryptococcal Morphogenesis. | |
PLoS Genet A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. | |
mBio A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Julie (Verified Customer) (07-07-2021) | I was using this kit to try a co-IP. My IP protein is tagged to cherry, but the very first step of IP did not work so I could not test for an interaction with other protein candidates. The antibody I used works very well. As you can see, the protein of interest is in the input and unbound fractions - meaning the RFP-protein did not bind to the RFP-trap agarose beads. Disappointed. I used buffers that were in the range that was recommended. It would be helpful if exact starting amounts of ingredients for buffers were provided since I can't test multiple conditions with a sample that only 2 reactions. I'm sure there is a way to troubleshoot, but again, I can't test multiple conditions with just 2 reactions and brain tissue is precious.
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