ChromoTek RFP-Trap Agarose

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : rta

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind ~ 9 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Sci Rep

Annexin A7 is required for ESCRT III-mediated plasma membrane repair.

Authors - Stine Lauritzen Sønder
IP

mBio

The Unfolded Protein Response Regulates Pathogenic Development of Ustilago maydis by Rok1-Dependent Inhibition of Mating-Type Signaling.

Authors - Lara Schmitz
IP

Mol Autism

Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus.

Authors - Fatemeh Hassani Nia
IP

mBio

PAS Domain Protein Pas3 Interacts with the Chromatin Modifier Bre1 in Regulating Cryptococcal Morphogenesis.

Authors - Youbao Zhao
IP

PLoS Genet

A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis.

Authors - Manoj K Singh
IP

mBio

A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus.

Authors - E Keats Shwab

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Julie (Verified Customer) (07-07-2021)

I was using this kit to try a co-IP. My IP protein is tagged to cherry, but the very first step of IP did not work so I could not test for an interaction with other protein candidates. The antibody I used works very well. As you can see, the protein of interest is in the input and unbound fractions - meaning the RFP-protein did not bind to the RFP-trap agarose beads. Disappointed. I used buffers that were in the range that was recommended. It would be helpful if exact starting amounts of ingredients for buffers were provided since I can't test multiple conditions with a sample that only 2 reactions. I'm sure there is a way to troubleshoot, but again, I can't test multiple conditions with just 2 reactions and brain tissue is precious.

  • Applications: Other
  • Primary Antibody Dilution: IP: 1mg/ml, Western Blot: 1:1000
  • Cell Tissue Type: Cerebellar brain tissue expressing an cherry-tagged protein