ChromoTek RFP-Trap Agarose
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
Ready to use reagent
Specificity
mCherry, mRFP, mPlum
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
154
Cat no : rta
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 10 µL slurry bind ~ 9 µg of recombinant mCherry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS RFP-Trap Agarose (PDF) |
Protocol |
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Protocol RFP-Trap Agarose (PDF) |
Application notes |
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Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
Split Fluorescent Protein Technology (PDF) |
Brochure |
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Product brochure (PDF) |
RFP-Trap Specificity |
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Fluorescent protein specificity table (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) (PDF) |
Publications
Application | Title |
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mBio The Unfolded Protein Response Regulates Pathogenic Development of Ustilago maydis by Rok1-Dependent Inhibition of Mating-Type Signaling. | |
Mol Autism Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus. | |
mBio PAS Domain Protein Pas3 Interacts with the Chromatin Modifier Bre1 in Regulating Cryptococcal Morphogenesis. | |
PLoS Genet A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. | |
mBio A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus. |