ChromoTek iST RFP-Trap Kit for AP-MS sample preparation of RFP-fusion proteins

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

mCherry, mRFP, mPlum

Applications

IP, MS

Conjugate

Agarose

Cat no : rtak-iST

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind more than 9 µg of recombinant mCherry
Storage Buffer20% ethanol

Kit components

ComponentDescription
RFP-Trap Agarose 8 reactions plus 2 controls
Digest Enzyme Trypsin-mix to digest proteins.
Resuspend buffer Protease reconstitution buffer for enzymes.
Lyse buffer Denature, reduce and alkylate proteins.
Stop solution Stop the enzymatic activity.
Wash 1 buffer Clean up peptides from hydrophobic contaminants.
Wash 2 buffer Clean up peptides from hydrophilic contaminants.
Elution buffer Elute the peptides from the cartridge.
LC-Load load Load peptides on reversed-phase LC-MS column.
Cartridges Cartridge for 1 to 100 µg protein starting material.
Waste tubes Tube for collecting waste after washing steps.
Collection tubes Tube for collecting peptides after elution.
Adapters Enables placing a cartridge into a tube.
Caps Cap to optionally close the cartridge’s bottom.