ChromoTek iST RFP-Trap Kit for AP-MS sample preparation of RFP-fusion proteins

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

mCherry, mRFP, mPlum

Applications

IP, MS

Conjugate

Agarose

Cat no : rtak-iST

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/Target mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind more than 9 µg of recombinant mCherry
Storage Buffer20% ethanol

Kit components

ComponentDescription
RFP-Trap Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.