ChromoTek iST RFP-Trap Kit for AP-MS sample preparation of RFP-fusion proteins

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

mCherry, mRFP, mPlum

Applications

IP, MS

Conjugate

Agarose

Cat no : rtak-iST

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  Make the most of your time & gain about 40 hours with the iST_RFP-Trap Kit.

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Product Information

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

• Applications: IP, MS
• Clonality: VHH
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• Coupled VHH: Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind more than 9 µg of recombinant mCherry
• Storage Buffer: 20% ethanol

Kit components

Component	Description
RFP-Trap Agarose	8 reactions plus 2 controls
Digest	Enzyme Trypsin-mix to digest proteins.
Resuspend buffer	Protease reconstitution buffer for enzymes.
Lyse buffer	Denature, reduce and alkylate proteins.
Stop solution	Stop the enzymatic activity.
Wash 1 buffer	Clean up peptides from hydrophobic contaminants.
Wash 2 buffer	Clean up peptides from hydrophilic contaminants.
Elution buffer	Elute the peptides from the cartridge.
LC-Load load	Load peptides on reversed-phase LC-MS column.
Cartridges	Cartridge for 1 to 100 µg protein starting material.
Waste tubes	Tube for collecting waste after washing steps.
Collection tubes	Tube for collecting peptides after elution.
Adapters	Enables placing a cartridge into a tube.
Caps	Cap to optionally close the cartridge’s bottom.