ChromoTek iST RFP-Trap Kit for AP-MS sample preparation of RFP-fusion proteins
Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)
Cat no : rtak-iST
Synonyms
Validation Data Gallery
Product Information
Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)
Applications | IP, MS |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 10 µL slurry bind more than 9 µg of recombinant mCherry |
Storage Buffer | 20% ethanol |
Kit components
Component | Description |
---|---|
RFP-Trap Agarose | 8 reactions plus 2 controls |
Digest | Enzyme Trypsin-mix to digest proteins. |
Resuspend buffer | Protease reconstitution buffer for enzymes. |
Lyse buffer | Denature, reduce and alkylate proteins. |
Stop solution | Stop the enzymatic activity. |
Wash 1 buffer | Clean up peptides from hydrophobic contaminants. |
Wash 2 buffer | Clean up peptides from hydrophilic contaminants. |
Elution buffer | Elute the peptides from the cartridge. |
LC-Load load | Load peptides on reversed-phase LC-MS column. |
Cartridges | Cartridge for 1 to 100 µg protein starting material. |
Waste tubes | Tube for collecting waste after washing steps. |
Collection tubes | Tube for collecting peptides after elution. |
Adapters | Enables placing a cartridge into a tube. |
Caps | Cap to optionally close the cartridge’s bottom. |
Documentation
SDS |
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SDS iST RFP-Trap Kit (PDF) |
Protocol |
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Protocol iST RFP-Trap Kit (PDF) |
RFP-Trap Specificity |
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Fluorescent protein specificity table (PDF) |
Brochure |
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iST Nano-Trap Kit product brochure (PDF) |