ChromoTek iST RFP-Trap® IP-MS Sample Preparation Kit

Validation Data Gallery

• 

  Schematic workflow of iST Nano-Trap® Kit

• 

  Save up to 40 hours on your mass spec experiments with the iST Nano-Trap® Kits

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

Cat No. rtak-iST

Specificity

mCherry, mPlum, mRFP

Applications

IP, MS

Type

Nanobody

Conjugate

Agarose

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

---

Product Information

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

• Description: The iST RFP-Trap® Kit for processing RFP-fusion proteins and their interacting partners for mass spectrometry analysis: The kit includes the RFP-Trap for IP/Co-IP of RFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

  • Reliable dentification of RFP-fusion proteins & interacting partners by MS

  • Identification of even low abundant RFP-fusion proteins

  • Superior processing of IP samples into clean peptide mixtures

  • Intuitive protocol for reproducible results

• Applications: IP, MS
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 22.5 μg of recombinant RFP per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 5 nM
• Compatibility with mass spectrometry: The RFP-Trap® is optimized for on-bead digestion.
• Storage Buffer: 20% ethanol
• Storage Condition: Kit is shipped at ambient temperature. Upon receipt store RFP-Trap at 4°C and Digest at -20°C

Kit components

• Component: Description
• RFP-Trap Agarose: 8 reactions plus 2 controls
• Digest: Enzyme Trypsin-mix to digest proteins.
• Resuspend buffer: Protease reconstitution buffer for enzymes.
• Lyse buffer: Denature, reduce and alkylate proteins.
• Stop solution: Stop the enzymatic activity.
• Wash 1 buffer: Clean up peptides from hydrophobic contaminants.
• Wash 2 buffer: Clean up peptides from hydrophilic contaminants.
• Elution buffer: Elute the peptides from the cartridge.
• LC-Load load: Load peptides on reversed-phase LC-MS column.
• Cartridges: Cartridge for 1 to 100 µg protein starting material.
• Waste tubes: Tube for collecting waste after washing steps.
• Collection tubes: Tube for collecting peptides after elution.
• Adapters: Enables placing a cartridge into a tube.
• Caps: Cap to optionally close the cartridge’s bottom.

---