ChromoTek RFP-Trap Magnetic Agarose, kit

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

Specificity

mCherry, mPlum, mRFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Publications

9

Cat no : rtmak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  RFP-Trap Magnetic Agarose for immunoprecipitation of RFP and mCherry fusion proteins.I: Input, FT: Flow-through, B: Bound.

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Product Information

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

• Description: The RFP-Trap® Magnetic Agarose Kit contains RFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of RFP-fusion proteins and their interacting factors.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 22.5 μg of recombinant RFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 5 nM
• Compatibility with mass spectrometry: The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631363
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component	Description
RFP-Trap® Magnetic Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

RFP-Trap Agarose kit

RFP-Trap Agarose

RFP-Trap Magnetic Agarose

RFP-Trap Magnetic Particles M-270 Kit

RFP-Trap Magnetic Particles M-270

Publications

Application	Title
IP	Mol Neurodegener; Nuclear import receptors are recruited by FG-nucleoporins to rescue hallmarks of TDP-43 proteinopathy; Authors - Bilal Khalil
IP	Commun Biol; βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.; Authors - Sayan Ghosh
IP	Commun Biol; βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.; Authors - Peng Shang
IP	mBio; CDC50 Orthologues in Plasmodium falciparum Have Distinct Roles in Merozoite Egress and Trophozoite Maturation.; Authors - Avnish Patel
IP	Cell Rep; RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss.; Authors - Stylianos Kosmidis
	Nucleic Acids Res; The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress; Authors - Zhe Han