ChromoTek RFP-Trap Magnetic Agarose, kit

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat no : rtmak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 40 µm
Binding capacity 10 µL slurry bind ~8 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
RFP-Trap® Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Cell Rep

RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss.

Authors - Stylianos Kosmidis
IP

Commun Biol

βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Authors - Sayan Ghosh
IP

Commun Biol

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Authors - Peng Shang