ChromoTek RFP-Trap Magnetic Agarose

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

Ready to use reagent

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Publications

44

Cat no : rtma

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  RFP-Trap Magnetic Agarose for immunoprecipitation of RFP and mCherry fusion proteins. I: Input, FT: Flow-through, B: Bound.

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Product Information

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• Coupled VHH: Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
• Conjugate: Magnetic Agarose
• Beads: ~ 40 µm
• Binding capacity: 10 µL slurry bind ~8 µg of recombinant mCherry
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Methods Mol Biol; Fluorescent protein specific Nanotraps to study protein-protein interactions and histone-tail peptide binding.; Authors - Garwin Pichler
IP	Cell Rep; Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint.; Authors - Thomas Wild
IP	Nat Plants; Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex in Arabidopsis.; Authors - Bailong Zhang
IP	Dev Biol; Shep interacts with posttranscriptional regulators to control dendrite morphogenesis in sensory neurons.; Authors - Eugenia C Olesnicky
IP	Mol Cell Biol; Truncated Adenomatous Polyposis Coli Mutation Induces Asef-Activated Golgi Fragmentation.; Authors - Sang Bum Kim
ChIP	Endocrinology; Glucocorticoid Receptor-Tethered Mineralocorticoid Receptors Increase Glucocorticoid-Induced Transcriptional Responses.; Authors - Caroline A Rivers