ChromoTek RFP-Trap Magnetic Agarose

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat no : rtma

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 40 µm
Binding capacity10 µL slurry bind ~8 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Methods Mol Biol

Fluorescent protein specific Nanotraps to study protein-protein interactions and histone-tail peptide binding.

Authors - Garwin Pichler
IP

Cell Rep

Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint.

Authors - Thomas Wild
IP

Nat Plants

Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex in Arabidopsis.

Authors - Bailong Zhang
IP

mBio

A PAS Protein Directs Metabolic Reprogramming during Cryptococcal Adaptation to Hypoxia.

Authors - Youbao Zhao
IP

Nat Plants

Evolutionarily conserved hierarchical gene regulatory networks for plant salt stress response.

Authors - Ting-Ying Wu
IP

Mol Cell Biochem

DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.

Authors - Shigeki Suzuki