ChromoTek RFP-Trap Magnetic Agarose
The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.
Ready to use reagent
Specificity
mCherry, mRFP, mPlum
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
Cat no : rtma
Synonyms
Validation Data Gallery
Product Information
The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Magnetic Agarose |
Beads | ~ 40 µm |
Binding capacity | 10 µL slurry bind ~8 µg of recombinant mCherry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS RFP-Trap Magnetic Agarose (PDF) |
Protocol |
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Protocol RFP-Trap Magnetic Agarose (PDF) |
Application notes |
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Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
Brochure |
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Product brochure (PDF) |
RFP-Trap Specificity |
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Fluorescent protein specificity table (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) (PDF) |
White papers |
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Split Fluorescent Protein Technology (PDF) |
Publications
Application | Title |
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Methods Mol Biol Fluorescent protein specific Nanotraps to study protein-protein interactions and histone-tail peptide binding. | |
Cell Rep Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint. | |
Nat Plants Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex in Arabidopsis. | |
Dev Biol Shep interacts with posttranscriptional regulators to control dendrite morphogenesis in sensory neurons. | |
Mol Cell Biol Truncated Adenomatous Polyposis Coli Mutation Induces Asef-Activated Golgi Fragmentation. | |
Endocrinology Glucocorticoid Receptor-Tethered Mineralocorticoid Receptors Increase Glucocorticoid-Induced Transcriptional Responses. |