ChromoTek RFP-Trap® Magnetic Particles M-270 Kit
RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Cat no : rtdk
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Applications | IP, CoIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
GeneID (NCBI) | 0 |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Magnetic Particles M-270 |
Beads | ~ 2.8 µm (Magnetic, high throughput-compatible) |
Binding capacity | 10 µL slurry bind ~ 0.5 µg of recombinant mCherry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
Component | Description |
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Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
Wash buffer | Removal of unwanted proteins, peptides, etc. |
Dilution buffer | Dilution of cell lysate |
Elution buffer | For acidic elution |
RFP-Trap Magnetic Particles M-270 | 20 reactions (500 µL) |
Documentation
SDS |
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SDS RFP-Trap Magnetic Particles M-270 Kit |
PROTOCOL |
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PROTOCOL RFP-Trap Magnetic Particles M-270 Kit |
RFP-Trap Specificity |
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Fluorescent protein specificity table |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
Brochure |
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RFP-Trap product brochure |