ChromoTek RFP-Trap® Magnetic Particles M-270, Kit for Immunoprecipitation

Validation Data Gallery

• 

  Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

• 

  Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

• 

  Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Cat No. rtdk

Publications(1)

Specificity

mCherry, mPlum, mRFP

Applications

IP, CoIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Synonyms

RFP, RFP tag, RFP-tag, RFP-Trap, RFP-Trap® Magnetic Particles M-270, RFP-Trap® Magnetic Particles M-270 Kit, rtdk, rtdk-20

---

Product Information

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

• Description: The RFP-Trap® Magnetic Particles M-270 Kit contains RFP-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of RFP-fusion proteins and their interacting factors.

  • IP and Co-IP of RFP-tagged proteins without size limitation

  • Automation and high throughput applications

  • Magnetic separation with easy and efficient washing of RFP-Trap Magnetic Particles M-270

  • Very high affinity (KD=5 nM) to bind even low abundant proteins

  • No heavy & light antibody chains, short incubation (5-30 min)

  • Extraordinary binding, also under harsh conditions

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 1.25 μg of recombinant mCherry per 25 μL bead slurry
• Conjugate: Magnetic Particles M-270, size: 2.8 µm; high throughput-compatible
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 0.5 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 5 nM
• Compatibility with mass spectrometry: The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2861253
• Storage Buffer: PBS with 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

• Component: Description
• Lysis buffer: Optimized for cytoplasmatic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution
• RFP-Trap Magnetic Particles M-270: 20 reactions (500 µL)

---

Publications

Application	Title
IP	Science; DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans; Authors - Yisui Xia