ChromoTek RFP-Trap® Magnetic Particles M-270 Kit

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP

Conjugate

Magnetic Particles M-270

Cat no : rtdk

Synonyms

RFP, RFP tag, RFP-tag, RFP-Trap, RFP-Trap® Magnetic Particles M-270, RFP-Trap® Magnetic Particles M-270 Kit, rtdk, rtdk-20

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Validation Data Gallery

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  Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

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  Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

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  Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

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Product Information

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

• Applications: IP, CoIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• GeneID (NCBI): 0
• Coupled VHH: Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
• Conjugate: Magnetic Particles M-270
• Beads: ~ 2.8 µm (Magnetic, high throughput-compatible)
• Binding capacity: 10 µL slurry bind ~ 0.5 µg of recombinant mCherry
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 1x PBS, Preservative: 0.09 % sodium azide
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution
RFP-Trap Magnetic Particles M-270	20 reactions (500 µL)