ChromoTek RFP-Trap Agarose, kit

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

5

Cat no : rtak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  Immunoprecipitation of mCherry fusion with RFP-Trap. I: Input, FT: Flow-through, B: Bound.

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Product Information

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: VHH
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• Coupled VHH: Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind ~ 9 µg of recombinant mCherry
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
RFP-Trap® Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Publications

Application	Title
IP	J Cell Sci; Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis.; Authors - Giorgio Belloni
CoIP	Open Biol; Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster.; Authors - Stefano Sechi
IP	Autophagy; Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae.; Authors - Yi Zhen Deng
IP	Mol Cell; ATG4 family proteins drive phagophore growth independently of the LC3/GABARAP lipidation system.; Authors - Thanh Ngoc Nguyen
IP	Science; Neuropilin-1 is a host factor for SARS-CoV-2 infection.; Authors - James L Daly