Validation Data Gallery
Product Information
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
| Description | The RFP-Trap® Agarose Kit contains RFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of RFP-fusion proteins and their interacting factors.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Short incubation time (30 min) |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 22.5 μg of recombinant RFP per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 10 mM DTT, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 5 nM |
| Compatibility with mass spectrometry | The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631362 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| RFP-Trap® Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| rtak_SDS_RFP-Trap® Agarose Kit (EN) |
| Datasheet |
|---|
| Protocol RFP-Trap Agarose Kit (PDF) |
| Application notes |
|---|
| Split Fluorescent Protein Technology (PDF) |
| Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
| RFP-Trap Specificity |
|---|
| Fluorescent protein specificity table (PDF) |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (IP) (PDF) |
Publications
| Application | Title |
|---|---|
Mol Cell ATG4 family proteins drive phagophore growth independently of the LC3/GABARAP lipidation system. | |
Autophagy Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae. | |
Open Biol Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster. | |
J Cell Sci Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis. | |
PLoS One The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ziqiao (Verified Customer) (06-10-2022) | The eluted proteins were much purified but only weaknesses is that the amount was very limited. The acid elution method might be optimized.
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