ChromoTek RFP-Trap Agarose, kit

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : rtak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind ~ 9 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
RFP-Trap® Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
CoIP

PLoS One

The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading.

Authors - Jisca Majolée
IP

J Cell Sci

Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis.

Authors - Giorgio Belloni
CoIP

Open Biol

Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster.

Authors - Stefano Sechi
IP

Autophagy

Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae.

Authors - Yi Zhen Deng
IP

Mol Cell

ATG4 family proteins drive phagophore growth independently of the LC3/GABARAP lipidation system.

Authors - Thanh Ngoc Nguyen
IP

Science

Neuropilin-1 is a host factor for SARS-CoV-2 infection.

Authors - James L Daly