ChromoTek RFP-Trap Agarose, kit

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

Specificity

mCherry, mPlum, mRFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : rtak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

DescriptionThe RFP-Trap® Agarose Kit contains RFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of RFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Short incubation time (30 min)

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity22.5 μg of recombinant RFP per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility10 mM DTT, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 5 nM
Compatibility with mass spectrometryThe RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631362
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
RFP-Trap® Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
CoIP

PLoS One

The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading.

Authors - Jisca Majolée
IP

J Cell Sci

Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis.

Authors - Giorgio Belloni
CoIP

Open Biol

Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster.

Authors - Stefano Sechi
IP

Autophagy

Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae.

Authors - Yi Zhen Deng
IP

Mol Cell

ATG4 family proteins drive phagophore growth independently of the LC3/GABARAP lipidation system.

Authors - Thanh Ngoc Nguyen
IP

Science

Neuropilin-1 is a host factor for SARS-CoV-2 infection.

Authors - James L Daly

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ziqiao (Verified Customer) (06-10-2022)

The eluted proteins were much purified but only weaknesses is that the amount was very limited. The acid elution method might be optimized.

  • Applications: Immunoprecipitation,Protein purification
  • Cell Tissue Type: HEK293T