ChromoTek RFP-Trap Agarose, kit
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
Specificity
mCherry, mRFP, mPlum
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : rtak
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 5 nM |
| Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
| Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Binding capacity | 10 µL slurry bind ~ 9 µg of recombinant mCherry |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| RFP-Trap® Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| SDS RFP-Trap Agarose Kit (PDF) |
| Protocol |
|---|
| Protocol RFP-Trap Agarose Kit (PDF) |
| Application notes |
|---|
| Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
| Split Fluorescent Protein Technology (PDF) |
| Brochure |
|---|
| Product brochure (PDF) |
| RFP-Trap Specificity |
|---|
| Fluorescent protein specificity table (PDF) |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (IP) (PDF) |
Publications
| Application | Title |
|---|---|
J Cell Sci Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis. | |
Open Biol Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster. | |
Autophagy Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae. | |