ChromoTek RFP-Trap Agarose, kit

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

6

Cat no : rtak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  Immunoprecipitation of mCherry fusion with RFP-Trap.I: Input, FT: Flow-through, B: Bound.

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Product Information

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• Coupled VHH: Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind ~ 9 µg of recombinant mCherry
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
RFP-Trap® Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Publications

Application	Title
CoIP	PLoS One; The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading.; Authors - Jisca Majolée
IP	J Cell Sci; Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis.; Authors - Giorgio Belloni
CoIP	Open Biol; Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster.; Authors - Stefano Sechi
IP	Autophagy; Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae.; Authors - Yi Zhen Deng
IP	Mol Cell; ATG4 family proteins drive phagophore growth independently of the LC3/GABARAP lipidation system.; Authors - Thanh Ngoc Nguyen
IP	Science; Neuropilin-1 is a host factor for SARS-CoV-2 infection.; Authors - James L Daly