ChromoTek RFP-Trap Agarose, kit
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
Specificity
mCherry, mRFP, mPlum
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : rtak
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 10 µL slurry bind ~ 9 µg of recombinant mCherry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
Component | Description |
---|---|
RFP-Trap® Agarose | 20 reactions (500 µL) |
Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
Wash buffer | Removal of unwanted proteins, peptides, etc. |
Dilution buffer | Dilution of cell lysate |
Elution buffer | For acidic elution |
Documentation
SDS |
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SDS RFP-Trap Agarose Kit (PDF) |
Protocol |
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Protocol RFP-Trap Agarose Kit (PDF) |
Application notes |
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Split Fluorescent Protein Technology (PDF) |
Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
Brochure |
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Product brochure (PDF) |
RFP-Trap Specificity |
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Fluorescent protein specificity table (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) (PDF) |
Publications
Application | Title |
---|---|
PLoS One The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading. | |
J Cell Sci Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis. | |
Open Biol Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster. | |
Autophagy Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae. | |
Mol Cell ATG4 family proteins drive phagophore growth independently of the LC3/GABARAP lipidation system. | |