Validation Data Gallery
![HeLa cells transiently transfected with
MannosidaseII-tdTomato (Golgi).
Primary antibody: [5F8] 1:1,000
Secondary antibody: goat anti-rat Alexa Fluor® 647
Fixation: 3.7 % PFA
Scale bar: 10 µm](https://www.ptglab.com/products/pictures/Result-5f8.png "HeLa cells transiently transfected with
MannosidaseII-tdTomato (Golgi).
Primary antibody: [5F8] 1:1,000
Secondary antibody: goat anti-rat Alexa Fluor® 647
Fixation: 3.7 % PFA
Scale bar: 10 µm")
Product Information
Rat monoclonal [5F8] to Red Fluorescent Proteins (RFP)
| Description | RFP rat monoclonal [5F8] for IF, ELISA |
| Applications | IF, WB, IP, FC, ELISA |
| Specificity/Target | mOrange, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, tdTomato, mKate2, mPlum For the complete list, please click here: Fluorescent protein specificity table antibodies |
| Recommended Dilution | Immunofluorescence: 1:1,000 |
| Host/IsoType | Rat/IgG2a |
| Class | Monoclonal |
| Type | Primary Antibody |
| Immunogen | UniProtKB: Q9U6Y8 |
| Clonality | Monoclonal |
| Clone No. | 5F8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A or G purified |
| RRID | AB_2336064 |
| Storage Buffer | PBS, 50% glycerol, preservative: 0.09% sodium azide. |
| Storage Condition | Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment. |
| Size | 20 μL; 150 μL |
| Background | Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a rat monoclonal antibody raised against RFP, reactive to a variety of RFP derivates, including mOrange, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, tdTomato, mKate2, and mPlum. |
Documentation
| SDS |
|---|
| 5f8_SDS_rat anti RFP Monoclonal antibody (5F8) (EN) |
| Datasheet |
|---|
| rat anti RFP Monoclonal antibody (5F8) Datasheet |
| ANTIBODY SPECIFICITY |
|---|
| Fluorescent protein specificity table antibodies |
Publications
| Application | Title |
|---|---|
Nature Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche. | |
Cancer Cell Tumor-initiating stem cells fine-tune the plasticity of neutrophils to sculpt a protective niche | |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Prabha (Verified Customer) (07-25-2025) | The antibody 5f8 binds very specific and the efficiency of immunodetection is excellent. Our lab is using this antibody for the past 6 years and we highly recommend it our neighboring labs too.
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FH Paulina (Verified Customer) (04-24-2025) | Endogenous mScarlet from CRISPR knock-in of a low-expression protein was detected with the 5f8 antibody. Antibody works nicely. Fixation: 2%PFA, triton permeabilization, primary antibody diluted 1:100, secondary antibody 1:200 anti-rat AF488-conjugated.
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FH Anzy (Verified Customer) (08-13-2024) | Works well against mScarlet-i
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FH Prabha (Verified Customer) (09-11-2023) | The RFP antibody 5F8 is really excellent. Immunodetection shows specific detection without any background signal.
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FH Ramzi (Verified Customer) (01-24-2023) | I have tested the anti 5f8 Rat and the secondary I used anti-GFP Rat 488. I used vibratome brain sections from creER2 tomato DsRed mice. I tested in 2 different ratio 1:200 and 1:1000 both worked very will and this is the images I took.
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