RFPL1/2/3 Polyclonal antibody

• 

  WB analysis of HeLa using 13215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13215-1-AP (RFPL1/2/3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 13215-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 13215-1-AP (RFPL1/2/3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

RFPL1/2/3 Polyclonal Antibody for WB, ELISA

Cat No. 13215-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

ret finger protein like 3, RFPL3

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Tested Applications

• Positive WB detected in: HeLa cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

13215-1-AP targets RFPL1/2/3 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4090

  Product name: Recombinant human RFPL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-288 aa of BC031689

  Sequence: MAALFQEASSCPVCSDYLEKPMSLECGCTVCLKCINSLQKEPHGEDLLCCCCSMVSQRNKIRPNRQLERLVSHIKELEPKMKKILQMNPRMRKFQVDMTLDADTANNFLLISDDLRSVRSGLITQNRQDLAERFDVSVCILGSPRFTCGRHYWEVDVGTSTEWDLGVCRESVHCKGKIQLTTELGFWTVSLRDGSRLSASTVPLTFLLVDRKLQRVGIFLDMGMQNVSFFDAESGSHVYTFRSVSAEEPLRPFLAPSIPPNGDQGVLSICPLMNSGTTDAPVRPGEAK

• Full Name: ret finger protein-like 3
• Calculated Molecular Weight: 317 aa, 35 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC031689
• Gene Symbol: RFPL3
• Gene ID (NCBI): 10738
• RRID: AB_2877925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75679
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Oncol Rep; EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.; Authors - Chuan Lin