RFPL4B-Specific Polyclonal antibody

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  IHC staining of rat testis using 20207-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20207-1-AP (RFPL4B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 20207-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RFPL4B-Specific antibody (20207-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RFPL4B-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 20207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

RFPL4B, RNF211, RING finger protein 211, Ret finger protein-like 4B, ret finger protein like 4B

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Tested Applications

• Positive IHC detected in: rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20207-1-AP targets RFPL4B-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ret finger protein-like 4B
• Calculated Molecular Weight: 30 kDa
• GenBank Accession Number: NM_001013734
• Gene Symbol: RFPL4B
• Gene ID (NCBI): 442247
• RRID: AB_3669329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZWI9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFPL4B also named as RNF211. This antibody is specific to RFPL4B. RFPL4B may enable metal ion binding activity.