RFTN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RFTN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24289-1-AP

Synonyms

Raftlin, PIG9, PIB10, MIG2, KIAA0084

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Validation Data Gallery

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  WB analysis of HeLa using 24289-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 24289-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

24289-1-AP targets RFTN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFTN1 fusion protein Ag17264
• Full Name: raftlin, lipid raft linker 1
• Calculated Molecular Weight: 578 aa, 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC100667
• Gene Symbol: RFTN1
• Gene ID (NCBI): 23180
• RRID: AB_2879474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).

Publications

Species	Application	Title
human	IF,IP	Angiogenesis; Raftlin is recruited by neuropilin-1 to the activated VEGFR2 complex to control proangiogenic signaling.; Authors - Asha L Bayliss
human,mouse	WB	Pathol Res Pract; RFTN1 facilitates gastric cancer progression by modulating AKT/p38 signaling pathways.; Authors - Chaowei Deng ; KD Validated
human	WB	Alzheimers Dement; Amyloid futures in the expanding pathology of brain aging and dementia; Authors - Max A Thorwald