RFTN2 Polyclonal antibody

RFTN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17854-1-AP

Synonyms

C2orf11, Raft linking protein 2, Raftlin 2, raftlin family member 2, RFTN2

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Validation Data Gallery

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  WB analysis using 17854-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17854-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17854-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 17854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse brain tissue, mouse testis tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17854-1-AP targets RFTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFTN2 fusion protein Ag12307
• Full Name: raftlin family member 2
• Calculated Molecular Weight: 501 aa, 56 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC111069
• Gene Symbol: RFTN2
• Gene ID (NCBI): 130132
• RRID: AB_2300934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.