Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19893-1-AP targets RFWD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13728 Product name: Recombinant human RFWD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 427-774 aa of BC002574 Sequence: SQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE Predict reactive species |
| Full Name | ring finger and WD repeat domain 3 |
| Calculated Molecular Weight | 774 aa, 85 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC002574 |
| Gene Symbol | RFWD3 |
| Gene ID (NCBI) | 55159 |
| RRID | AB_10639521 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PCD5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RFWD3 antibody 19893-1-AP | Download protocol |
| IHC protocol for RFWD3 antibody 19893-1-AP | Download protocol |
| WB protocol for RFWD3 antibody 19893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res DOCK7 protects against replication stress by promoting RPA stability on chromatin. | ||
Front Oncol The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.
| ||
Front Med Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer. | ||
Front Oncol RFWD3 modulates response to platinum chemotherapy and promotes cancer associated phenotypes in high grade serous ovarian cancer | ||
Histol Histopathol RFWD3 acts as a tumor promotor in the development and progression of bladder cancer | ||
bioRxiv The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.
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