RFWD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RFWD3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19893-1-AP

Synonyms

RFWD3, RING finger protein 201, RNF201

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Validation Data Gallery

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  WB analysis of mouse lung using 19893-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19893-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human testis using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human colon using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 19893-1-AP

  Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 19893-1-AP

  Immunofluorescent analysis of Hela cells, using RFWD3 antibody 19893-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:200
• Immunofluorescence (IF): IF : 1:20-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 19893-1-AP is RFWD3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFWD3 fusion protein Ag13728
• Full Name: ring finger and WD repeat domain 3
• Calculated Molecular Weight: 774 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC002574
• Gene Symbol: RFWD3
• Gene ID (NCBI): 55159
• RRID: AB_10639521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
human	WB,IF	Front Oncol; The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.; Authors - Guochao Liu ; KD Validated
human	IHC	Front Med; Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer.; Authors - Yanfei Zhang
human	IHC	Histol Histopathol; RFWD3 acts as a tumor promotor in the development and progression of bladder cancer; Authors - Peng Jiang