RFX3 Polyclonal antibody

RFX3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14784-1-AP

Synonyms

Regulatory factor X 3, RFX3, Transcription factor RFX3

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Validation Data Gallery

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  WB analysis of HeLa using 14784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14784-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14784-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14784-1-AP

  IP result of anti-RFX3 (IP:14784-1-AP, 4ug; Detection:14784-1-AP 1:500) with HeLa cells lysate 1400ug.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

14784-1-AP targets RFX3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFX3 fusion protein Ag6413
• Full Name: regulatory factor X, 3 (influences HLA class II expression)
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC067778
• Gene Symbol: RFX3
• Gene ID (NCBI): 5991
• RRID: AB_2178917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	ChIP	Nat Commun; Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.; Authors - Karen R Mifsud
mouse	WB	Aging (Albany NY); RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.; Authors - Chan Chen