RFX5 Polyclonal antibody

RFX5 Polyclonal Antibody for WB, ELISA

Cat No. 12137-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

DNA-binding protein RFX5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 12137-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12137-1-AP (RFX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

HeLa cells, Jurkat cells, K-562 cells, Raji cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

12137-1-AP targets RFX5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2783 Product name: Recombinant human RFX5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 268-616 aa of BC017471 Sequence: NGLENPEGGAHKKPERLAQPPKDLEARTGAGPLARGERKKSVVESSAPGANNLQVNALVARLPLLLPRAPRSLIPPIPVSPPILAPRLSSGALKVATLPLSSRAGAPPAAVPIINMILPTVPALPGPGPGPGRAPPGGLTQPRGTENREVGIGGDQGPHDKGVKRTAEVPVSEASGQAPPAKAAKQDIEDTASDAKRKRGRPRKKSGGSGERNSTPLKSAAAMESAQSSRLPWETWGSGGEGNSAGGAERPGPMGEAEKGAVLAQGQGDGTVSKGGRGPGSQHTKEAEDKIPLVPSKVSVIKGSRSQKEAFPLAKGEVDTAPQGNKDLKEHVLQSSLSQEHKDPKATPP Predict reactive species
Full Name	regulatory factor X, 5 (influences HLA class II expression)
Calculated Molecular Weight	616 aa, 65 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC017471
Gene Symbol	RFX5
Gene ID (NCBI)	5993
RRID	AB_2179039
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48382
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for RFX5 antibody 12137-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Sci Rep RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A. Authors - Dong-Bo Chen KO Validated View Article
human	WB	Chin Med J (Engl) Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis. Authors - Dong-Bo Chen View Article
mouse,human	WB	Cell Death Dis Targeting MED23 inhibits hepatocellular carcinoma development by suppressing compensatory proliferation and facilitating ROS-mediated cell death. Authors - Xiaying Zhao View Article