RGR Polyclonal antibody

RGR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11904-1-AP

Synonyms

RGR

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Validation Data Gallery

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  WB analysis of human heart using 11904-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11904-1-AP (RGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11904-1-AP

  IP result of anti-RGR (IP:11904-1-AP, 4ug; Detection:11904-1-AP 1:300) with PC-3 cells lysate 4000ug.

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  IHC staining of mouse retina using 11904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse retina using 11904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11904-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11904-1-AP (RGR antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: mouse retina tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11904-1-AP targets RGR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGR fusion protein Ag2552
• Full Name: retinal G protein coupled receptor
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa, 26 kDa
• GenBank Accession Number: BC008094
• Gene Symbol: RGR
• Gene ID (NCBI): 5995
• RRID: AB_2179498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGR is a G protein-coupled receptor preferentially expressed at high levels in the retinal pigment epithelium (RPE) and Mueller cells of the neural retina. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. RGR acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The gene of PGR may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms.