RGS11 Polyclonal antibody

RGS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26132-1-AP

Synonyms

RGS11, RS11

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Validation Data Gallery

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  WB analysis of HL-60 using 26132-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26132-1-AP (RGS11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 26132-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26132-1-AP (RGS11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 26132-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26132-1-AP (RGS11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26132-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26132-1-AP (RGS11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, rat brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26132-1-AP is RGS11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS11 fusion protein Ag23562
• Full Name: regulator of G-protein signaling 11
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC153019
• Gene Symbol: RGS11
• Gene ID (NCBI): 8786
• RRID: AB_2880394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.