RGS17 Polyclonal antibody

RGS17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12549-1-AP

Synonyms

RGSZ2, RGS 17, Regulator of G-protein signaling 17, hRGS17

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Validation Data Gallery

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  WB analysis using 12549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12549-1-AP (RGS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12549-1-AP (RGS17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12549-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 12549-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

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  IHC staining of human cerebellum using 12549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

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  IHC staining of human cerebellum using 12549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

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  IF Staining of MCF-7 using 12549-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12549-1-AP (RGS17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, PC-3 cells, SH-SY5Y cells, mouse brain tissue, mouse lung tissue, MDA-MB-231 cells, NCI-H1299 cells, SK-BR-3 cells
• Positive IHC detected in: human testis tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12549-1-AP targets RGS17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS17 fusion protein Ag3399
• Full Name: regulator of G-protein signaling 17
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC013117
• Gene Symbol: RGS17
• Gene ID (NCBI): 26575
• RRID: AB_2179954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Signal; Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma.; Authors - Eugene Sokolov
rat	WB,IF	Sci Rep; Regulator of G protein signaling 17 represents a novel target for treating cisplatin induced hearing loss.; Authors - Asmita Dhukhwa
human	WB, IHC	J Cancer; Deregulation of RGS17 Expression Promotes Breast Cancer Progression.; Authors - Yuhua Li