RGS3 Monoclonal antibody

RGS3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D6A3

Cat no : 66790-1-Ig

Synonyms

C2PA, FLJ20370, FLJ31516, FLJ90496, PDZ RGS3, RGP3, RGS3

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 66790-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66790-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66790-1-Ig targets RGS3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RGS3 fusion protein Ag3177
• Full Name: regulator of G-protein signaling 3
• Calculated Molecular Weight: 1198 aa, 132 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC018072
• Gene Symbol: RGS3
• Gene ID (NCBI): 5998
• RRID: AB_2882134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGS3 belongs to a family of proteins which share the core regulator of G-protein signalling (RGS) domain and function as GTPase-activating proteins (GAPs) for the α subunit of heterotrimeric G-proteins. RGS3 has been reported to inhibit the Gai-and Gaq-mediated signaling pathways in the cardiovascular system, including those acting via the cardiovascular signaling molecules angiotensin II (Ang II), sphingosine 1-phosphate and endothelin-1. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear.

Publications

Species	Application	Title
mouse	WB	Transl Cancer Res; Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1; Authors - Xiao Hu