RGS8 Recombinant monoclonal antibody, PBS Only (Capture)

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• RGS8 Recombinant monoclonal antibody, PBS Only (Capture) (88102-1-PBS)

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  WB analysis using 88102-1-RR (same clone as 88102-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 88102-1-RR (same clone as 88102-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 88102-1-RR (same clone as 88102-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP03353-2

  The mean RGS8 concentration was determined to be 88.79 ng/mL in mouse liver extract based on a 8.40 mg/mL extract load, 4.65 ng/mL in mouse skin extract based on a 0.90 mg/mL extract load and 41.22 ng/mL in rat brain extract based on a 3.90 mg/mL extract load.

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  Sandwich ELISA standard curve of MP03353-2

  Sandwich ELISA standard curve of MP03353-2, Human RGS8 Recombinant Matched Antibody Pair - PBS only. 88102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26535. 88102-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

RGS8 Uni-rAb^® Recombinant Antibody for WB, IF-P, Sandwich ELISA, Indirect ELISA

Cat No. 88102-1-PBS

Clone No.260122C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, Sandwich ELISA, Indirect ELISA

Synonyms

Regulator of G-protein signaling 8

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Product Information

88102-1-PBS targets RGS8 as part of a matched antibody pair:

MP03353-2: 88102-1-PBS capture and 88102-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26535

  Product name: Recombinant human RGS8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-198 aa of BC069718

  Sequence: NKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS

• Full Name: regulator of G-protein signaling 8
• Calculated Molecular Weight: 198 aa, 23 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC069718
• Gene Symbol: RGS8
• Gene ID (NCBI): 85397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P57771
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.