RGS9 Polyclonal antibody

RGS9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17970-1-AP

Synonyms

PERRS, RGS9, RGS9L

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Validation Data Gallery

• 

  WB analysis of mouse brain using 17970-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17970-1-AP (RGS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17970-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17970-1-AP (RGS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17970-1-AP targets RGS9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS9 fusion protein Ag11976
• Full Name: regulator of G-protein signaling 9
• Calculated Molecular Weight: 622 aa, 71 kDa
• Observed Molecular Weight: 58 kDa, 70-75 kDa
• GenBank Accession Number: BC022504
• Gene Symbol: RGS9
• Gene ID (NCBI): 8787
• RRID: AB_2178508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGS9 is a member of the RGS family of signaling proteins that suppress the activity of G proteins by promoting their deactivation. There are two splice isoforms of RGS9 with quite different properties and patterns of expression. RGS9-1 is mainly found in the eye and is involved in regulation of phototransduction in rod and cone cells of the retina, while RGS9-2 is is expressed specifically in the striatum, a brain region involved in controlling movement, motivation, mood and addition. This antibody got about 58kD in the mouse eye and 70kD in the mouse brain in western blotting, both in line with their expected masses. Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS).

Publications

Species	Application	Title
human	WB	Genes (Basel); Extended Phenotyping and Functional Validation Facilitate Diagnosis of a Complex Patient Harboring Genetic Variants in MCCC1 and GNB5 Causing Overlapping Phenotypes.; Authors - Zhuo Shao