RGSL1 Polyclonal antibody

RGSL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25836-1-AP

Synonyms

RGSL, RGSL1, RGSL2

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Validation Data Gallery

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  WB analysis of mouse brain using 25836-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25836-1-AP (RGSL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25836-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 25836-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25836-1-AP is RGSL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGSL1 fusion protein Ag20269
• Full Name: regulator of G-protein signaling like 1
• Calculated Molecular Weight: 1076 aa, 126 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC142944
• Gene Symbol: RGSL1
• Gene ID (NCBI): 353299
• RRID: AB_2880263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGSL1, also named as RGSL and RGSL2, belongs to the RSG family. RGSL1 is involved in the G-protein signaling regulation with a suggested role in prostate carcinogenesis (PMID: 28662289). RGSL1 has 6 isoforms with the molecular mass of 47-63, 71 and 126 kDa. RGSL1 may interact with other markers in the development of breast cancer (PMID: 21135262).