RHAMM,Lgals9 Polyclonal antibody

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  WB analysis using 15819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15819-1-AP (RHAMM,Lgals9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15819-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15819-1-AP (RHAMM,Lgals9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15819-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15819-1-AP (RHAMM,Lgals9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

RHAMM,Lgals9 Polyclonal Antibody for WB, ELISA

Cat No. 15819-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Hmmr, RHAMM

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Tested Applications

• Positive WB detected in: mouse spleen tissue, NIH/3T3 cells, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15819-1-AP targets RHAMM,Lgals9 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hyaluronan mediated motility receptor (RHAMM)
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 32 kDa, 70 kDa
• GenBank Accession Number: NM_012964
• Gene Symbol: RHAMM
• Gene ID (NCBI): 25460
• RRID: AB_2137234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9WUF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMMR, also named as RHAMM, is a hyaluronan receptor expressed in various tissues. It is involved in regulation of focal contacts and subsequent motility, thus to play function in wound healing and tumor metastasis. The protein was expressed as a 70kd one in many tissues.