RHBDF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RHBDF2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 23181-1-AP

Synonyms

RHBDF2, RHBDL5, RHBDL6, Rhomboid 5 homolog 2, Rhomboid family member 2

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Validation Data Gallery

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  IHC staining of human colon cancer using 23181-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23181-1-AP (RHBDF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 23181-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23181-1-AP (RHBDF2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 23181-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 23181-1-AP (RHBDF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Product Information

The immunogen of 23181-1-AP is RHBDF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHBDF2 fusion protein Ag19710
• Full Name: rhomboid 5 homolog 2 (Drosophila)
• Calculated Molecular Weight: 856 aa, 97 kDa
• GenBank Accession Number: BC016034
• Gene Symbol: RHBDF2
• Gene ID (NCBI): 79651
• RRID: AB_2879224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Int; RHBDF2 gene functions are correlated to facilitated renal clear cell carcinoma progression.; Authors - Lei Wang ; KD Validated
human	WB,IHC	FEBS Open Bio; RHBDF2 is correlated with immune infiltrates in hepatocellular carcinoma and may have potential as a biomarker; Authors - Hanjuan Gong
human	WB,IHC	Biotechnol Genet Eng Rev; The prognostic value of RHBDF2 in Pan-Cancer, and its correlation with cell Adhesion of Hepatocellular Carcinoma; Authors - Hanjuan Gong
monkey	WB	J Virol; The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1; Authors - Feifei Diao
pig	WB,IP	Vet Microbiol; Porcine reproductive and respiratory syndrome virus infection activates ADAM17 to induce inflammatory responses; Authors - Jiao Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (02-20-2019)

This antibody cannot be used for WB blot. It gives multiple bands, and the band around the expected molecular weight was still there in iRhom2 (another name for Rhbdf2) KO or KD samples.

• Applications: Western Blot,