Tested Applications
| Positive IP detected in | mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27977-1-AP targets RHNO1 in applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14789 Product name: Recombinant human C12orf32 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-241 aa of BC005106 Sequence: HRLMPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS Predict reactive species |
| Full Name | chromosome 12 open reading frame 32 |
| Calculated Molecular Weight | 238 aa, 27 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC005106 |
| Gene Symbol | C12orf32 |
| Gene ID (NCBI) | 83695 |
| RRID | AB_3742268 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSD3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RHNO1 (Rad9-Hus1-Rad1 Interacting Nuclear Orphan 1) is a protein associated with the DNA replication stress (DRS) response and DNA repair. It interacts with the 9-1-1 checkpoint clamp and TopBP1 to activate the ATR/Chk1 signaling pathway, thereby maintaining genomic integrity. Additionally, RHNO1 has been identified as a key facilitator of theta-mediated end joining (TMEJ), a DNA repair mechanism related to cancer progression and chemotherapy resistance. In cancer research, RHNO1 is considered a potential therapeutic target due to its high expression in tumor cells and its regulatory role in cell proliferation and survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for RHNO1 antibody 27977-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
