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RHOA Monoclonal antibody
RHOA Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66733-1-Ig
|Positive WB detected in||HeLa cells, HEK-293 cells, Jurkat cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66733-1-Ig targets RHOA in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
|Tested Reactivity||Human, mouse, pig, rat|
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RHOA fusion protein Ag1141|
|Full Name||ras homolog gene family, member A|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC005976|
|Gene ID (NCBI)||387|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.
Cell Death Differ
Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserines.
Clin Sci (Lond)
The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.
J Biol Chem
SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion
Front Mol Biosci
CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.
GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer.
Exp Cell Res
RALY may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer.