|Positive WB detected in||HeLa cells, HEK-293 cells, Jurkat cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66733-1-Ig targets RHOA in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
|Tested Reactivity||Human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RHOA fusion protein Ag1141|
|Full Name||ras homolog gene family, member A|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC005976|
|Gene ID (NCBI)||387|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.
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