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  • KD/KO Validated

RHOB Polyclonal antibody

RHOB Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14326-1-AP

Synonyms

ARH6, ARHB, h6, MST081, MSTP081, Rho cDNA clone 6, RHOB, RHOH6



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, SH-SY5Y cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14326-1-AP targets RHOB in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOB fusion protein Ag5593
Full Name ras homolog gene family, member B
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC066954
Gene symbol RHOB
Gene ID (NCBI) 388
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RHOB antibody 14326-1-APDownload protocol
IHC protocol for RHOB antibody 14326-1-APDownload protocol
IP protocol for RHOB antibody 14326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biomed Res Int

PMID: 31011573

Authors - Qing Ma
  • KD Validated
humanWB

Open Life Sci

The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer.

Authors - Xinxin Hao
humanWB

Exp Ther Med

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Authors - Min Li
humanWB

Biochemistry

A Complete Survey of RhoGDI Targets Reveals Novel Interactions with Atypical Small GTPases.

Authors - Ana Masara Binti Ahmad Mokhtar
humanWB,IHC

PLoS One

RhoB Acts as a Tumor Suppressor That Inhibits Malignancy of Clear Cell Renal Cell Carcinoma.

Authors - Weihao Chen
humanWB

Exp Cell Res

RALY may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer.

Authors - Guanchu Song