RHOBTB1 Recombinant monoclonal antibody

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  WB analysis using 83342-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83342-1-RR (RHOBTB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83342-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOBTB1 antibody (83342-1-RR, Clone: 240253F10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83342-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83342-1-RR against Human RHOBTB1 were performed. The affinity constant is 0.502 nM.

RHOBTB1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83342-1-RR

Clone No.240253F10

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

KIAA0740, RHOBTB1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U-251 cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83342-1-RR targets RHOBTB1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3365

  Product name: Recombinant human RHOBTB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-344 aa of BC032848

  Sequence: MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQAD

• Full Name: Rho-related BTB domain containing 1
• Calculated Molecular Weight: 696 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC032848
• Gene Symbol: RHOBTB1
• Gene ID (NCBI): 9886
• RRID: AB_3671003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94844
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation (PMID: 31789920).