Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12290-1-AP targets RHOF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2943 Product name: Recombinant human RHOF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC018208 Sequence: MDAPGALAQTAAPGPGRKELKIVIVGDGGCGKTSLLMVYSQGSFPEHYAPSVFEKYTASVTVGSKEVTLNLYDTAGQEDYDRLRPLSYQNTHLVLICYDVMNPTSYDNVLIKWFPEVTHFCRGIPMVLIGCKTDLRKDKEQLRKLRAAQLEPITYMQVGRGQDPGAQPWL Predict reactive species |
| Full Name | ras homolog gene family, member F (in filopodia) |
| Calculated Molecular Weight | 211 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC018208 |
| Gene Symbol | RHOF |
| Gene ID (NCBI) | 54509 |
| RRID | AB_10644126 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RHOF antibody 12290-1-AP | Download protocol |
| IP protocol for RHOF antibody 12290-1-AP | Download protocol |
| WB protocol for RHOF antibody 12290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RHOF antibody (Cat# 12290-1-AP) has 6 total citations published in EPMA Journal, Cell Biosciences, International Journal of Molecular Sciences, iScience, Journal of Biological Chemistry, and Virchows Archiv. Research fields span lung adenocarcinoma, hepatocellular carcinoma, pancreatic cancer, acute myeloid leukemia, Wnt/Rho signaling, and primary mediastinal large B-cell lymphoma, with applications in WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
EPMA J Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies | ||
Cell Biosci SIAH2-EPHB6 axis enhances filopodia formation in hepatocellular carcinoma cells by regulating RHOF. | ||
Int J Mol Sci An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer | ||
iScience RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance
| ||
J Biol Chem Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression | ||
Virchows Arch High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma |
