MIRO1 Polyclonal antibody

MIRO1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21560-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RHOT1, ARHT1, EC:3.6.5.-, hMiro 1, MIRO 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 21560-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21560-1-AP (MIRO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 21560-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21560-1-AP (MIRO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

21560-1-AP targets MIRO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15605 Product name: Recombinant human RHOT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 277-618 aa of BC060781 Sequence: DDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKGLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR Predict reactive species
Full Name	ras homolog gene family, member T1
Calculated Molecular Weight	618 aa, 71 kDa
Observed Molecular Weight	70-80 kDa
GenBank Accession Number	BC060781
Gene Symbol	MIRO1
Gene ID (NCBI)	55288
RRID	AB_3669369
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IXI2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for MIRO1 antibody 21560-1-AP	Download protocol
WB protocol for MIRO1 antibody 21560-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
rat	WB	Bioact Mater Advanced nerve regeneration enabled by neural conformal electronic stimulators enhancing mitochondrial transport Authors - Hao Bai View Article
		Adv Sci (Weinh) MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis Authors - La Zhang View Article
	WB	Nat Cell Biol Mitochondrial-derived vesicles drive budding-type fission of damaged lysosomes Authors - Yuanchun Luo View Article