RHOV Polyclonal antibody

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• RHOV Polyclonal antibody (26620-1-AP)

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• KD/KO Validated

RHOV Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26620-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

ARHV, CDC42 like GTPase 2, CDC42-like GTPase 2, Chp, GTP binding protein like 2

• 

  WB analysis of rat lung using 26620-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26620-1-AP (RHOV Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 26620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 26620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: rat lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26620-1-AP targets RHOV in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24331

  Product name: Recombinant human RHOV protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 200-236 aa of BC112945

  Sequence: SAILSAIEHKARLEKKLNAKGVRTLSRCRWKKFFCFV

• Full Name: ras homolog gene family, member V
• Calculated Molecular Weight: 236 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC112945
• Gene Symbol: RHOV
• Gene ID (NCBI): 171177
• RRID: AB_2880576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96L33
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-RHOV antibody (Cat# 26620-1-AP) has 7 total citations published in Cancer Research, International Journal of Biological Sciences, American Journal of Pathology, Frontiers in Oncology, bioRxiv, and Cancer Cell International. Research fields include ovarian cancer peritoneal metastasis, lung adenocarcinoma progression and EGFR-TKI resistance, pancreatic ductal adenocarcinoma, and Rho GTPase signaling pathways. Applications cited are primarily WB and IHC in human samples.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Res; RHOV is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal Metastasis.; Authors - Amal T Elhaw ; KO Validated
human	WB	Int J Biol Sci; RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.; Authors - Deyu Zhang
human	IHC	Am J Pathol; Ras Homolog Family Member V Promotes Pancreatic Ductal Adenocarcinoma Progression.; Authors - Shang Wu
human	WB,IHC	Front Oncol; Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma.; Authors - Hongjin Chen ; KD Validated
		bioRxiv; RHOV is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal Metastasis; Authors - Amal T Elhaw
		Cancer Cell Int; LLPS-based classification and a novel prognostic signature reveal NRF1 as a therapeutic target in pancreatic cancer.; Authors - Weishen Wang