RHPN1 Polyclonal antibody

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  WB analysis of human brain using 13460-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13460-1-AP (RHPN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 13460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13460-1-AP (RHPN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13460-1-AP

  IP result of anti-RHPN1 (IP:13460-1-AP, 4ug; Detection:13460-1-AP 1:300) with Jurkat cells lysate 3400ug.

RHPN1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13460-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

GTP Rho binding protein 1, KIAA1929, ODF5, RHOPHILIN, Rhophilin 1, RHPN, RHPN1

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Tested Applications

• Positive WB detected in: A375 cells, human brain tissue, Jurkat cells, PC-3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13460-1-AP targets RHPN1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3933

  Product name: Recombinant human RHPN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 321-670 aa of BC025767

  Sequence: RLVHRTMAQPPVHDYVPVSWTALVHVKAEYFRSLAHYHVAMALCDGSPATEGELPTHEQVFLQPPTSSKPRGPVLPQELEERRQLGKAHLKRAILGQEEALRLHALCRVLREVDLLRAVISQTLQRSLAKYAELDREDDFCEAAEAPDIQPKTHQKPEARMPRLSQGKGPDIFHRLGPLSVFSAKNRWRLVGPVHLTRGEGGFGLTLRGDSPVLIAAVIPGSQAAAAGLKEGDYIVSVNGQPCRWWRHAEVVTELKAAGEAGASLQVVSLLPSSRLPSLGDRRPVLLGPRGLLRSQREHGCKTPASTWASPRPLLNWSRKAQQGKTGGCPQPCAPVKPAPPSSLKHPGWP

• Full Name: rhophilin, Rho GTPase binding protein 1
• Calculated Molecular Weight: 670 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC025767
• Gene Symbol: RHPN1
• Gene ID (NCBI): 114822
• RRID: AB_2179682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TCX5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.