Tested Applications
| Positive WB detected in | COLO 320 cells, HepG2 cells |
| Positive IHC detected in | human colon tissue, human stomach tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12671-1-AP targets RHPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3353 Product name: Recombinant human RHPN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 385-686 aa of BC036447 Sequence: LYDHMPEGLTPLATLKNDQQRRQLGKSHLRRAMAHHEESVREASLCKKLRSIEVLQKVLCAAQERSRLTYAQHQEEDDLLNLIDAPSVVAKTEQEVDIILPQFSKLTVTDFFQKLGPLSVFSANKRWTPPRSIRFTAEEGDLGFTLRGNAPVQVHFLDPYCSASVAGAREGDYIVSIQLVDCKWLTLSEVMKLLKSFGEDEIEMKVVSLLDSTSSMHNKSATYSVGMQKTYSMICLAIDDDDKTDKTKKISKKLSFLSWGTNKNRQKSASTLCLPSVGAARPQVKKKLPSPFSLLNSDSSWY Predict reactive species |
| Full Name | rhophilin, Rho GTPase binding protein 2 |
| Calculated Molecular Weight | 686 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC036447 |
| Gene Symbol | RHPN2 |
| Gene ID (NCBI) | 85415 |
| RRID | AB_2877872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUC4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| IHC protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| WB protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RHPN2 Polyclonal antibody (Cat# 12671-1-AP) has 3 citations published in Science Signaling, Cell Cycle, and Journal of Biochemical and Molecular Toxicology. The research fields span neural differentiation in murine stem cells, ovarian cancer cell proliferation and invasion via the integrin β1/NF-κB pathway, and pancreatic cancer proliferation, metastasis, and angiogenesis through circ_0000284/RHPN2/miR-1179 axis.
| Species | Application | Title |
|---|---|---|
Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. | ||
Cell Cycle P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.
| ||
J Biochem Mol Toxicol Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179 |
