RIBC2 Polyclonal antibody

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  WB analysis using 15272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15272-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15272-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15272-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIBC2 antibody (15272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RIBC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15272-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C22orf11, RIB43A-like with coiled-coils protein 2

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse testis tissue, human cervical cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15272-1-AP targets RIBC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIBC2 fusion protein Ag7385
• Full Name: RIB43A domain with coiled-coils 2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC003024
• Gene Symbol: RIBC2
• Gene ID (NCBI): 26150
• RRID: AB_2179714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4K1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIBC2 is a microtubule protein and is part of the double microtubules (DMTs) decorated with dynamic proteins in the ciliary axons. It is mainly located in the microtubules of cilia and is involved in flagellated sperm motility(PMID: 40265983).