RIBC2 Polyclonal antibody

• 

  WB analysis of human brain using 15272-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15272-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15272-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIBC2 antibody (15272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RIBC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15272-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RIB43A-like with coiled-coils protein 2, C22orf11

---

Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: mouse testis tissue, human cervical cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15272-1-AP targets RIBC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIBC2 fusion protein Ag7385
• Full Name: RIB43A domain with coiled-coils 2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC003024
• Gene Symbol: RIBC2
• Gene ID (NCBI): 26150
• RRID: AB_2179714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4K1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.