RIC8A Polyclonal antibody

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  WB analysis of human brain using 18707-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18707-1-AP (RIC8A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18707-1-AP (RIC8A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18707-1-AP (RIC8A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 18707-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIC8A antibody (18707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RIC8A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18707-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein Ric-8A, RIC8, synembryn, Synembryn A, Synembryn-A

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: rat brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

18707-1-AP targets RIC8A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC011821
• Gene Symbol: RIC8A
• Gene ID (NCBI): 60626
• RRID: AB_2179831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Dev Dyn; Nucleotide exchange factor RIC-8 is indispensable in mammalian early development.; Authors - T?nissoo Tambet T