Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
17790-1-AP targets RIC8B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11888 Product name: Recombinant human RIC8B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-495 aa of BC034220 Sequence: ELQGLPLLTQILESAFSIKWTDEYESAIDHNGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEATTLDELPSNKTAEKETVLKNNTMVYNGMNMEAIHVLLNFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDG Predict reactive species |
| Full Name | resistance to inhibitors of cholinesterase 8 homolog B (C. elegans) |
| Calculated Molecular Weight | 495 aa, 56 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC034220 |
| Gene Symbol | RIC8B |
| Gene ID (NCBI) | 55188 |
| RRID | AB_2285188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVN3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ric8, also known as synembryn, is a guanine nucleotide exchange factor (GEF) that enhances variegated G protein signaling pathways, including Gα(q) and Gα(s) regulation of neurotransmission, Gα(i)-dependent mitotic spindle positioning during (asymmetric) cell division, and Gα(olf)-dependent odorant receptor signaling (PMID: 21467038). In mammals, two Ric8 homologues have been reported (Ric8A and Ric8B). Ric8B is able to potentiate G(s)/G(olf) signaling (PMID: 15829631; 16754875; 20133939).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIC8B antibody 17790-1-AP | Download protocol |
| IHC protocol for RIC8B antibody 17790-1-AP | Download protocol |
| WB protocol for RIC8B antibody 17790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling. | ||
Sci Rep The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia. | ||
PLoS One Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. |
