RIM2-Specific Polyclonal antibody

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  WB analysis of L02 using 20093-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20093-1-AP (RIM2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RIM2-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20093-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA0751, OBOE, Rab 3 interacting molecule 2, Rab 3 interacting protein 3, RAB3IP3, RIM 2, RIM2, RIM2-Specific, RIMS2

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20093-1-AP targets RIM2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: regulating synaptic membrane exocytosis 2
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: NM_001100117
• Gene Symbol: RIMS2
• Gene ID (NCBI): 9699
• RRID: AB_10666429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UQ26
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIMS2, also named as KIAA0751, RAB3IP3 and RIM2, is a rab effector which involved in exocytosis. RIMS2 may act as scaffold protein. This antibody is specific to RIMS2. It recognizes all the isoforms except isoform7 of RIMS2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	bioRxiv; Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers; Authors - Brianna Hnath

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody works well in detecting Rims2 in mouse tissue samples.

• Applications: Western Blot
• Cell Tissue Type: Heart