RIN3 Polyclonal antibody

• 

  WB analysis of HepG2 using 12709-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12709-1-AP (RIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

RIN3 Polyclonal Antibody for WB, ELISA

Cat No. 12709-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RIN 3, Ras and Rab interactor 3

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12709-1-AP targets RIN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3390

  Product name: Recombinant human RIN3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 309-657 aa of BC025248

  Sequence: SSTEMLQEIRTMMTQLKSYLLQSTELKALVDPALHSEEELEAIVESALYKCVLKPLKEAINSCLHQIHSKDGSLQQLKENQLVILATTTTDLGVTTSVPEVPMMEKFLQKFTSMHKAYSPEKKISILLKTCKLIYDSMALGNPGKPYGADDFLPVLMYVLARSNLTEMLLNVEYMMELMDPALQLGEGSYYLTTTYGALEHIKSYDKITVTRQLSVEVQDSIHRWERRRTLNKARASRSSVQDFICVSYLEPEQQARTLASRADTQAQALCAQCAEKFAVERPQAHRLFVLVDGRCFQLADDALPHCIKGYLLRSEPKRDFHFVYRPLDGGGGGGGSPPCLVVREPNFL

• Full Name: Ras and Rab interactor 3
• Calculated Molecular Weight: 985 aa, 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC025248
• Gene Symbol: RIN3
• Gene ID (NCBI): 79890
• RRID: AB_10638315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TB24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; Identification and validation of diagnostic biomarkers for temporal lobe epilepsy related to ferroptosis and potential therapeutic targets; Authors - Dai Shi
mouse	WB	Int J Mol Sci; The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model; Authors - Valentina Latina