RING1 Polyclonal antibody

RING1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15037-1-AP

Synonyms

Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1

---

Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 15037-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 15037-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15037-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15037-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 15037-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, BxPC-3 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

15037-1-AP targets RING1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RING1 fusion protein Ag6763
• Full Name: ring finger protein 1
• Calculated molecular weight: 42 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC002922
• Gene symbol: RING1
• Gene ID (NCBI): 6015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.