Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | rat stomach tissue, human placenta tissue, mouse cerebellum tissue, human stomach cancer tissue, rat skin tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17222-1-AP targets RIOK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11057 Product name: Recombinant human RIOK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 219-568 aa of BC006104 Sequence: DRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK Predict reactive species |
| Full Name | RIO kinase 1 (yeast) |
| Calculated Molecular Weight | 568 aa, 66 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC006104 |
| Gene Symbol | RIOK1 |
| Gene ID (NCBI) | 83732 |
| RRID | AB_2284990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| IHC protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| IP protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| WB protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIOK1 Polyclonal antibody (Cat# 17222-1-AP) has 7 citations. These appear in journals including Nature Cancer, Molecular Cell, Cell Reports, Biomedicines, iScience, and Cancer Research and Treatment. The associated research fields span hepatocellular carcinoma, ribosome biogenesis and degradation, mRNA processing, glioblastoma, Ras-driven EMT and invasion, and breast cancer progression. Applications cited include Western blot, immunoprecipitation, IHC, and IF, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Nat Cancer RIOK1 phase separation restricts PTEN translation via stress granules activating tumor growth in hepatocellular carcinoma | ||
Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. | ||
iScience Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion. |
Reviews
What customers say
The single review for this RIOK1 antibody (17222-1-AP) is a 5-star rating. The reviewer reported reliable and reproducible results in both Western blot and co-immunoprecipitation assays using HCT 116 cells at a 1:1000 dilution. They highlighted the antibody's good specificity and robust overall performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marta (Verified Customer) (05-08-2026) | We obtained very reliable and reproducible results using this RIOK1 antibody in both Western blot and co-immunoprecipitation assays. The antibody shows good specificity and robust performance.
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