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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85668-5-RR targets RIOK1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RIOK1 fusion protein Ag11057 Predict reactive species |
| Full Name | RIO kinase 1 (yeast) |
| Calculated Molecular Weight | 568 aa, 66 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC006104 |
| Gene Symbol | RIOK1 |
| Gene ID (NCBI) | 83732 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BRS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIOK1 antibody 85668-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
