Tested Applications
| Positive WB detected in | Caco-2 cells, HCT 116 cells |
| Positive IHC detected in | mouse brain tissue, human stomach cancer tissue, mouse lung tissue, rat lung tissue, mouse pancreas tissue, rat stomach tissue, mouse ovary tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17410-1-AP targets RIOK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9975 Product name: Recombinant human RIOK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-553 aa of BC000953 Sequence: QMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE Predict reactive species |
| Full Name | RIO kinase 2 (yeast) |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC000953 |
| Gene Symbol | RIOK2 |
| Gene ID (NCBI) | 55781 |
| RRID | AB_2178093 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVS4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rio-kinase 2 (RIOK2) is an atypical serine-threonine kinase that participates in the nuclear export and maturation steps of the pre-40S ribosomal complex to facilitate cytoplasmic translation. RIOK2 binds the pre-40S subunit and promotes its nuclear export by interaction with the CRM1 chaperone protein (PMID: 36661680, 28499923). High expression of RIOK2 has been linked to decreased overall survival in non-small cell lung cancer.. RIOK2's kinase activ DD-RIPK1-caspase-8 complex from lysosome to ER (PMID: 41249793).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIOK2 antibody 17410-1-AP | Download protocol |
| IHC protocol for RIOK2 antibody 17410-1-AP | Download protocol |
| WB protocol for RIOK2 antibody 17410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIOK2 polyclonal antibody (Cat# 17410-1-AP) has 1 citation to date. It was used in an immunofluorescence study published in the International Journal of Surgery (IF: 9), investigating how Blautia coccoides-derived 5Z-dodecenoic acid attenuates chronic psychological stress-induced gastric cancer progression in human samples. The associated research field is gastrointestinal oncology and gut microbiome–cancer interactions.
| Species | Application | Title |
|---|---|---|
Int J Surg Gut blautia coccoides-derived 5Z-dodecenoic acid attenuates chronic psychological stress-induced gastric cancer progression. |
