RIOK3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85449-5-RR (same clone as 85449-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85449-5-RR (RIOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 85449-5-RR (same clone as 85449-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 85449-5-RR (same clone as 85449-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 85449-5-RR (same clone as 85449-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85449-5-RR (same clone as 85449-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 85449-5-RR (same clone as 85449-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85449-5-RR (same clone as 85449-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85449-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85449-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85449-5-RR against Human RIOK3 were performed. The affinity constant is below 1 pM.

RIOK3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 85449-5-PBS

Clone No.242996A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242996A6, EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3

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Product Information

85449-5-PBS targets RIOK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4497

  Product name: Recombinant human RIOK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 215-516 aa of BC039729

  Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

• Full Name: RIO kinase 3 (yeast)
• Calculated Molecular Weight: 516 aa, 60 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC039729
• Gene Symbol: RIOK3
• Gene ID (NCBI): 8780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14730
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.