RIPK2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85760-4-RR (same clone as 85760-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85760-4-RR (RIPK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85760-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 85760-4-RR (same clone as 85760-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85760-4-RR (RIPK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85760-4-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85760-4-RR (same clone as 85760-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RIPK2 antibody (85760-4-RR, Clone: 250069D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85760-4-PBS in a different storage buffer formulation.

RIPK2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 85760-4-PBS

Clone No.250069D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CARD3, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARDIAK, CCK

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Product Information

85760-4-PBS targets RIPK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0909

  Product name: Recombinant human RIPK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 221-531 aa of BC004553

  Sequence: EVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPS

• Full Name: receptor-interacting serine-threonine kinase 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 46-55 kDa, 61-72 kDa
• GenBank Accession Number: BC004553
• Gene Symbol: RIPK2
• Gene ID (NCBI): 8767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43353
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.