RLBP1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85697-1-RR (same clone as 85697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  WB analysis of ARPE-19 using 85697-1-RR (same clone as 85697-1-PBS)

  ARPE-19 cells were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (85697-1-RR, Clone: 242989D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

RLBP1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 85697-1-PBS

Clone No.242989D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

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Product Information

85697-1-PBS targets RLBP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7602

  Product name: Recombinant human RLBP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-317 aa of BC004199

  Sequence: MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRLQYPELFDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDENILPSDFGGTLPKYDGKAVAEQLFGPQAQAENTAF

• Full Name: retinaldehyde binding protein 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004199
• Gene Symbol: RLBP1
• Gene ID (NCBI): 6017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12271
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).