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  • KD/KO Validated

RLIM Polyclonal antibody

RLIM Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16121-1-AP

Synonyms

NY REN 43, R LIM, RING finger protein 12, RLIM, RNF12



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16121-1-AP targets RLIM in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RLIM fusion protein Ag9077
Full Name ring finger protein, LIM domain interacting
Calculated Molecular Weight 624 aa, 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC013357
Gene Symbol RLIM
Gene ID (NCBI) 51132
RRIDAB_2181967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RLIM (RING finger LIM domain-binding protein), also known as RNF12 (RING finger protein 12) or NY-REN-43, is a 624 amino acid RING-H2 zinc finger protein that is involved in protein ubiquitinylation and subsequent degradation. Expressed in a variety of tissues, RLIM binds to the LIM domain of various proteins and functions as a protein ligase that negatively co-regulates LIM homeodomain (LIM-HD) transcription factors. Through its interaction with Sin3A, a component of the histone deacetylase corepressor complex, RLIM is able to recruit the corepressor complex to LIM-HD proteins, thereby inhibiting LIM-HD transcription. In addition to recruiting the deacetylase complex to LIM-HD proteins, RLIM is able to bind to, ubiquinate and subsequently degrade CLIM proteins, which function as positive co-regulators of LIM-HD transcription factors. RLIM contains one RING-type zinc finger and is implicated in renal cell carcinoma. The calcualted molecular weight of RLIM is 69 kDa, but modified RLIM is about 70-75 kDa.

Protocols

Product Specific Protocols
WB protocol for RLIM antibody 16121-1-APDownload protocol
IP protocol for RLIM antibody 16121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Cell Death Dis

RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis.

Authors - Yongsheng Huang
mouseWB

Elife

MOF-associated complexes ensure stem cell identity and Xist repression.

Authors - Tomasz Chelmicki
humanWB

Oncotarget

PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.

Authors - Chao Li
human,mouseWB,IHC,IF,CoIP

J Mol Cell Biol

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Authors - Yuwei Li
  • KD Validated
humanWB

Oncotarget

RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21.

Authors - Yongsheng Huang