RLIM Polyclonal antibody

• Featured Product
• KD/KO Validated

RLIM Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16121-1-AP

Synonyms

NY REN 43, R LIM, RING finger protein 12, RLIM, RNF12

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Validation Data Gallery

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  WB analysis of HeLa using 16121-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16121-1-AP (RLIM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16121-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16121-1-AP (RLIM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16121-1-AP

  IP result of anti-RLIM (IP:16121-1-AP, 4ug; Detection:16121-1-AP 1:500) with HeLa cells lysate 1520ug.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

16121-1-AP targets RLIM in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RLIM fusion protein Ag9077
• Full Name: ring finger protein, LIM domain interacting
• Calculated Molecular Weight: 624 aa, 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC013357
• Gene Symbol: RLIM
• Gene ID (NCBI): 51132
• RRID: AB_2181967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RLIM (RING finger LIM domain-binding protein), also known as RNF12 (RING finger protein 12) or NY-REN-43, is a 624 amino acid RING-H2 zinc finger protein that is involved in protein ubiquitinylation and subsequent degradation. Expressed in a variety of tissues, RLIM binds to the LIM domain of various proteins and functions as a protein ligase that negatively co-regulates LIM homeodomain (LIM-HD) transcription factors. Through its interaction with Sin3A, a component of the histone deacetylase corepressor complex, RLIM is able to recruit the corepressor complex to LIM-HD proteins, thereby inhibiting LIM-HD transcription. In addition to recruiting the deacetylase complex to LIM-HD proteins, RLIM is able to bind to, ubiquinate and subsequently degrade CLIM proteins, which function as positive co-regulators of LIM-HD transcription factors. RLIM contains one RING-type zinc finger and is implicated in renal cell carcinoma. The calcualted molecular weight of RLIM is 69 kDa, but modified RLIM is about 70-75 kDa.

Publications

Species	Application	Title
human	IP	Cell Death Dis; RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis.; Authors - Yongsheng Huang
mouse	WB	Elife; MOF-associated complexes ensure stem cell identity and Xist repression.; Authors - Tomasz Chelmicki
human	WB	Oncotarget; PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.; Authors - Chao Li
human,mouse	WB,IHC,IF,CoIP	J Mol Cell Biol; Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.; Authors - Yuwei Li ; KD Validated
human	WB	Oncotarget; RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21.; Authors - Yongsheng Huang