Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14630-1-AP targets RMI1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6208 Product name: Recombinant human RMI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC064937 Sequence: MNVTSIALRAETWLLAAWHVKVPPMWLEACINWIQEENNNVNLSQAQMNKQVFEQWLLTDLRDLEHPLLPDGILEIPKGELNGFYALQINSLVDVSQPAHSQIQKLRGKNTTNDLVTAEAQVTPKPWEAKPSRMLMLQLTDGIVQIQGMEYQPIPILHSDLPPGTKILIYGNISFRLGVLLLKPENVKVLGGEVDALLEEYAQEKVLARLIGEPDLVVSVIPNNSNENIPRVTDVLDPALGPSDEELLASLDENDELTANNDTSSERCFTTGSSSNTIPTRQSSFEPEFVISPRPKEEPSNLSIHVMDGELDDFSLEEALLLEETVQKEQMETKELQPLTFNRNADRSIER Predict reactive species |
| Full Name | RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC064937 |
| Gene Symbol | RMI1 |
| Gene ID (NCBI) | 80010 |
| RRID | AB_2301203 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9A7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RMI1 antibody 14630-1-AP | Download protocol |
| IP protocol for RMI1 antibody 14630-1-AP | Download protocol |
| WB protocol for RMI1 antibody 14630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RMI1 antibody (Cat# 14630-1-AP) has 16 citations across journals including Nature Communications, Molecular Cell, Nucleic Acids Research, Molecular Therapy, Cell Reports, Journal of Biological Chemistry, Oncology Reports, and DNA Cell Biology. Research fields span DNA repair, genome instability, RecQ helicase biology, Fanconi anemia pathway, topoisomerase IIIα function, R-loop resolution, telomere maintenance, and cancer progression (prostate, glioma). Applications include WB, IF, and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia.
| ||
Mol Cell Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
| ||
Nucleic Acids Res The SMC5/SMC6 complex is critical for resolving R-loop-induced transcription-replication conflicts. | ||
Mol Ther Improved Genome Editing through Inhibition of FANCM and Members of the BTR Dissolvase Complex. | ||
Cell Death Discov RMI1 facilitates repair of ionizing radiation-induced DNA damage and maintenance of genomic stability
| ||
