Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17559-1-AP targets RMND5A in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11751 Product name: Recombinant human RMND5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC047668 Sequence: MDQCVTVERELEKVLHKFSGYGQLCERGLEELIDYTGGLKHEILQSHGQDAELSGTLSLVLTQCCKRIKDTVQKLASDHKDIHSSVSRVGKAIDKNFDSDISSVGIDGCWQADSQRLLNEVMVEHFFRQGMLDVAEELCQESGLSVDPSQKEPFVELNRILEALKVRVLRPALEWAVSNREMLIAQNSSLEFKLHRLYFISLLMGGTTNQREALQYAKNFQPFALNHQKDIQVLMGSLVYLRQGIENSPYVHLLDANQWADICDIFTRDACALLGLSVESPLSVSFSAGCVALPALINIKAVIEQRQCTGVWNQKDELPIEVDLGKKCWYHSIFACPILRQQTTDNNPPMKLVCGHIISRDALNKMFNGSKLKCPYCPMEQSPGDAKQIFF Predict reactive species |
| Full Name | required for meiotic nuclear division 5 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 391 aa, 44 kDa |
| Observed Molecular Weight | 40-44 kDa |
| GenBank Accession Number | BC047668 |
| Gene Symbol | RMND5A |
| Gene ID (NCBI) | 64795 |
| RRID | AB_2878416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H871 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RMND5A antibody 17559-1-AP | Download protocol |
| WB protocol for RMND5A antibody 17559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RMND5A Polyclonal antibody (Cat# 17559-1-AP) has 3 citations. These appear in Molecular Cell, Cancers (Basel), and Chembiochem. The associated research fields include mTORC1-regulated proteasomal degradation of HMG-CoA synthase, colorectal cancer progression via MAPK signaling, and quantitative degradation proteomics for identifying E3 ligase substrates. All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway
| ||
Cancers (Basel) Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.
| ||
Reviews
What customers say
The single review for this antibody (used for Western Blot at 1:1000 on HEK293T cells) was rated 2 out of 5. The reviewer reported difficulty using it to screen CRISPR knockouts, noting numerous non-specific bands and a weak signal at the expected molecular weight. Overall, the feedback suggests the antibody may not be ideal for detecting knockout effects in this cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (09-27-2020) | I used this antibody to screen CRISPR-knock out, and found it difficult to do so (at least in HEK293T cells). There are so so many non-specific bands, and the right MW band was weak.
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