Featured Product
KD/KO Validated

RMND5A Polyclonal antibody

RMND5A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17559-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RMD5, Protein RMD5 homolog A, P44CTLH, EC:2.3.2.27, E3 ubiquitin-protein transferase RMND5A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis using 17559-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17559-1-AP (RMND5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 17559-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 17559-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 17559-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 17559-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 17559-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 17559-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (3)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells
Positive IHC detected in	human breast cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17559-1-AP targets RMND5A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11751 Product name: Recombinant human RMND5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC047668 Sequence: MDQCVTVERELEKVLHKFSGYGQLCERGLEELIDYTGGLKHEILQSHGQDAELSGTLSLVLTQCCKRIKDTVQKLASDHKDIHSSVSRVGKAIDKNFDSDISSVGIDGCWQADSQRLLNEVMVEHFFRQGMLDVAEELCQESGLSVDPSQKEPFVELNRILEALKVRVLRPALEWAVSNREMLIAQNSSLEFKLHRLYFISLLMGGTTNQREALQYAKNFQPFALNHQKDIQVLMGSLVYLRQGIENSPYVHLLDANQWADICDIFTRDACALLGLSVESPLSVSFSAGCVALPALINIKAVIEQRQCTGVWNQKDELPIEVDLGKKCWYHSIFACPILRQQTTDNNPPMKLVCGHIISRDALNKMFNGSKLKCPYCPMEQSPGDAKQIFF Predict reactive species
Full Name	required for meiotic nuclear division 5 homolog A (S. cerevisiae)
Calculated Molecular Weight	391 aa, 44 kDa
Observed Molecular Weight	40-44 kDa
GenBank Accession Number	BC047668
Gene Symbol	RMND5A
Gene ID (NCBI)	64795
RRID	AB_2878416
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H871
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for RMND5A antibody 17559-1-AP	Download protocol
WB protocol for RMND5A antibody 17559-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cancers (Basel) Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway. Authors - Chunyuan Tang KD Validated View Article
human	WB	Chembiochem Identifying E3 ligase substrates with quantitative degradation proteomics Authors - Victoria N Jordan View Article
human	WB	Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway Authors - Sang Ah Yi KO Validated View Article

Reviews

2.0 out of 5

Add a review

1 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

H (Verified Customer) (09-27-2020)

I used this antibody to screen CRISPR-knock out, and found it difficult to do so (at least in HEK293T cells). There are so so many non-specific bands, and the right MW band was weak.