Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17559-1-AP targets RMND5A in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11751 Product name: Recombinant human RMND5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC047668 Sequence: MDQCVTVERELEKVLHKFSGYGQLCERGLEELIDYTGGLKHEILQSHGQDAELSGTLSLVLTQCCKRIKDTVQKLASDHKDIHSSVSRVGKAIDKNFDSDISSVGIDGCWQADSQRLLNEVMVEHFFRQGMLDVAEELCQESGLSVDPSQKEPFVELNRILEALKVRVLRPALEWAVSNREMLIAQNSSLEFKLHRLYFISLLMGGTTNQREALQYAKNFQPFALNHQKDIQVLMGSLVYLRQGIENSPYVHLLDANQWADICDIFTRDACALLGLSVESPLSVSFSAGCVALPALINIKAVIEQRQCTGVWNQKDELPIEVDLGKKCWYHSIFACPILRQQTTDNNPPMKLVCGHIISRDALNKMFNGSKLKCPYCPMEQSPGDAKQIFF Predict reactive species |
| Full Name | required for meiotic nuclear division 5 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 391 aa, 44 kDa |
| Observed Molecular Weight | 40-44 kDa |
| GenBank Accession Number | BC047668 |
| Gene Symbol | RMND5A |
| Gene ID (NCBI) | 64795 |
| RRID | AB_2878416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H871 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RMND5A antibody 17559-1-AP | Download protocol |
| WB protocol for RMND5A antibody 17559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RMND5A Polyclonal antibody (Cat# 17559-1-AP) has 3 total citations published in Molecular Cell, Cancers (Basel), and Chembiochem. The associated research fields include protein degradation and ubiquitin-proteasome pathways (mTORC1-CTLH E3 ligase, Pro/N-degron pathway), colorectal cancer and MAPK signaling (LINC01234 oncopeptide MBOP), and quantitative degradation proteomics for identifying E3 ligase substrates. All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway
| ||
Cancers (Basel) Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.
| ||
Reviews
What customers say
One reviewer (H. An) used this antibody for Western Blot on HEK293T cells at a 1:1000 dilution to screen CRISPR knockouts. They reported difficulty with the application, noting numerous non-specific bands and a weak signal at the expected molecular weight. The review was rated 2 out of 5 stars (September 2020, lot 00009131).
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (09-27-2020) | I used this antibody to screen CRISPR-knock out, and found it difficult to do so (at least in HEK293T cells). There are so so many non-specific bands, and the right MW band was weak.
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