RNASE3 Polyclonal antibody

RNASE3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55338-1-AP

Synonyms

ECP, Eosinophil cationic protein, Ribonuclease 3, RNase 3, RNASE3, RNS3

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Validation Data Gallery

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  WB analysis of A549 using 55338-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55338-1-AP (RNASE3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55338-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55338-1-AP (RNASE3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 55338-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55338-1-AP (RNASE3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55338-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55338-1-AP (RNASE3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55338-1-AP

  Immunofluorescent analysis of HepG2 cells using 55338-1-AP (RNASE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

55338-1-AP targets RNASE3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribonuclease, RNase A family, 3 (eosinophil cationic protein)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: NM_002935
• Gene Symbol: RNASE3
• Gene ID (NCBI): 6037
• RRID: AB_2881312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Allergy; Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity; Authors - Jonathan G Medernach
mouse	IF	Cell Commun Signal; Allergen-induced CD11c + dendritic cell pyroptosis aggravates allergic rhinitis; Authors - Yue-Long Qiao
mouse	IHC	J Pharm Biomed Anal; Network pharmacology analysis and experimental validation of Xiao-Qing-Long-Tang's therapeutic effects against neutrophilic asthma; Authors - Zhifeng Chen