RNASE4 Polyclonal antibody

RNASE4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30732-1-AP

Synonyms

Ribonuclease 4, RNase 4, RNASE4, RNS4

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Validation Data Gallery

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  WB analysis using 30732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat lung using 30732-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 30732-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 30732-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human milk, THP-1 cells
• Positive IHC detected in: rat lung tissue, mouse bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30732-1-AP targets RNASE4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNASE4 fusion protein Ag29272
• Full Name: ribonuclease, RNase A family, 4
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC015520
• Gene Symbol: RNASE4
• Gene ID (NCBI): 6038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Ribonuclease 4 (RNase 4), one of the eight members of the human RNase A superfamily of endoribonucleases, is a uridine-specific endoribonuclease with a preference to cleave RNA after uridine residues prior to purines (PMID: 33818125). RNase 4 is a newly identified host defense peptide in the human kidney and bladder and kills uropathogenic E. coli (UPEC) and multi-drug resistant UPEC (PMID: 28955965).