Tested Applications
| Positive WB detected in | HeLa cells, U2OS cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 37 publications below |
| IHC | See 2 publications below |
| IF | See 20 publications below |
| ChIP | See 1 publications below |
Product Information
15606-1-AP targets RNASEH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNASEH1 fusion protein Ag7990 Predict reactive species |
| Full Name | ribonuclease H1 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC002973 |
| Gene Symbol | RNASEH1 |
| Gene ID (NCBI) | 246243 |
| RRID | AB_2238624 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60930 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
| IF protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
| IP protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. | ||
Cell Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy. | ||
Nature BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
| ||
Cell Res Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. | ||
Nat Cell Biol Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mulavini (Verified Customer) (09-23-2024) | Works good
|
FH R (Verified Customer) (06-15-2024) | Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells
|
FH Andrea (Verified Customer) (10-05-2023) | Strong signal, very good detection.
|
FH Huai-Chin (Verified Customer) (06-08-2019) | Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.
|
