• Featured Product
  • KD/KO Validated

RNASEH1 Polyclonal antibody

RNASEH1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 15606-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, U2OS cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15606-1-AP targets RNASEH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7990

Product name: Recombinant human RNASEH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-286 aa of BC002973

Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Predict reactive species
Full Name ribonuclease H1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002973
Gene Symbol RNASEH1
Gene ID (NCBI) 246243
RRIDAB_2238624
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Protocols

Product Specific Protocols
WB protocol for RNASEH1 antibody 15606-1-APDownload protocol
IF protocol for RNASEH1 antibody 15606-1-APDownload protocol
IP protocol for RNASEH1 antibody 15606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.

Authors - Aparna Gorthi
humanWB

Cell

Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.

Authors - Luciano E Marasco
humanWB

Nature

BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.

Authors - Vaibhav Bhatia
  • KD Validated
humanWB

Cell Res

Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.

Authors - Junyu Wu
mouseIF

Nat Cell Biol

Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.

Authors - Flavia Michelini
humanWB

Nat Commun

RNA-mediated double-strand break repair by end-joining mechanisms

Authors - Youngkyu Jeon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mulavini (Verified Customer) (09-23-2024)

Works good

  • Applications: Western Blot
FH

R (Verified Customer) (06-15-2024)

Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
FH

Andrea (Verified Customer) (10-05-2023)

Strong signal, very good detection.

  • Applications: Western Blot
FH

Huai-Chin (Verified Customer) (06-08-2019)

Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:3000 for Western, 1:500 for IHC, 1:200 for IF-IC
  • Cell Tissue Type: Mammary gland/ MCF7
Loading...
||
New chat Able
正在加载,请稍候...