RNASEH1 Polyclonal antibody

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  WB analysis using 15606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15606-1-AP

  WB result of RNASEH1 antibody (15606-1-AP; 1:3000; room temperature for 1.5 hours) with wild-type and RNASEH1 knockout HeLa cells.

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  IP experiment of HeLa using 15606-1-AP

  IP result of anti-RNASEH1 (IP:15606-1-AP, 4ug; Detection:15606-1-AP 1:3000) with HeLa cells lysate 1750 ug.

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  IF Staining of HeLa using 15606-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RNASEH1 antibody (15606-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

RNASEH1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 15606-1-AP

Publications(56)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 37 publications below
• IHC: See 2 publications below
• IF: See 20 publications below
• ChIP: See 1 publications below

Product Information

15606-1-AP targets RNASEH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7990

  Product name: Recombinant human RNASEH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-286 aa of BC002973

  Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

• Full Name: ribonuclease H1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC002973
• Gene Symbol: RNASEH1
• Gene ID (NCBI): 246243
• RRID: AB_2238624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60930
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.; Authors - Aparna Gorthi
human	WB	Cell; Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.; Authors - Luciano E Marasco
human	WB	Nature; BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.; Authors - Vaibhav Bhatia ; KD Validated
human	WB	Cell Res; Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.; Authors - Junyu Wu
mouse	IF	Nat Cell Biol; Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.; Authors - Flavia Michelini
human	WB	Nat Commun; RNA-mediated double-strand break repair by end-joining mechanisms; Authors - Youngkyu Jeon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mulavini (Verified Customer) (09-23-2024)

Works good

• Applications: Western Blot

FH

R (Verified Customer) (06-15-2024)

Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Andrea (Verified Customer) (10-05-2023)

Strong signal, very good detection.

• Applications: Western Blot

FH

Huai-Chin (Verified Customer) (06-08-2019)

Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:3000 for Western, 1:500 for IHC, 1:200 for IF-IC
• Cell Tissue Type: Mammary gland/ MCF7